A Homogeneous Fluorescence Polarization Immunoassay for the Determination of Cephalexin and Cefadroxil in Milk

被引：35

作者：

Zhang, Jing ^{[1
,2
]}

Wang, Zhanhui ^{[1
,2
]}

Mi, Tiejun ^{[1
,2
]}

Wenren, Lequn ^{[1
,2
]}

Wen, Kai ^{[1
,2
]}

机构：

[1] China Agr Univ, Coll Vet Med, Beijing 100093, Peoples R China

[2] Natl Reference Labs Vet Drug Residue, Beijing 100193, Peoples R China

来源：

FOOD ANALYTICAL METHODS | 2014年 / 7卷 / 04期

关键词：

Fluorescence polarization immunoassay; Monoclonal antibody; Cephalexin; Cefadroxil; Milk; LINKED-IMMUNOSORBENT-ASSAY; MONOCLONAL-ANTIBODY; IMMUNOANALYSIS SYSTEM; MASS-SPECTROMETRY; CHICKEN MUSCLE; HUMAN-PLASMA; SULFAMETHAZINE; ELISA; FOOD; FLUOROIMMUNOASSAY;

D O I：

10.1007/s12161-013-9695-4

中图分类号：

TS2 [食品工业];

学科分类号：

100403 [营养与食品卫生学];

摘要：

A new monoclonal antibody (mAb) against cephalexin (CEX), named 1F3, was produced by immunizing mice with immunogen CEX-keyhole limpet hemocyanin. Two fluorescein-labeled tracers (CEX-fluorescein isothiocyanate (FITC) and cefadroxil (CDX)-FITC) were synthesized and purified by thin-layer chromatography. A homogeneous fluorescence polarization immunoassay (FPIA) based on the new mAb and the selected tracer for the simultaneous determination of CEX and CDX was developed and optimized. The optimized FPIA provided a limit of detection of 1.8 and 2.3 ng ml(-1) for CEX and CDX and exhibited cross-reactivities of 100 and 63.16 % for CEX and CDX, respectively, and <1% for other 11 cephalosporins. Recoveries from fortified milk were ranged from 82 to 125 % with coefficient of variations of 3.2-14.2 %. The results indicated that the homogeneous FPIA we developed was enough sensitive to rapidly monitor CEX and CDX in milk.

引用

页码：879 / 886

页数：8

共 32 条

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