Bending elasticities of model membranes: Influences of temperature and sterol content

被引:260
作者
Meleard, P [1 ]
Gerbeaud, C [1 ]
Pott, T [1 ]
FernandezPuente, L [1 ]
Bivas, I [1 ]
Mitov, MD [1 ]
Dufourcq, J [1 ]
Bothorel, P [1 ]
机构
[1] BULGARIAN ACAD SCI,INST SOLID STATE PHYS,BU-1784 SOFIA,BULGARIA
关键词
D O I
10.1016/S0006-3495(97)78905-7
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Giant liposomes obtained by electroformation and observed by phase-contrast video microscopy show spontaneous deformations originating from Brownian motion that are characterized, in the case of quasispherical vesicles, by two parameters only, the membrane tension sigma and the bending elasticity k(c). For liposomes containing dimyristoyl phosphatidylcholine (DMPC) or a 10 mol% cholesterol/DMPC mixture, the mechanical property of the membrane, k(c), is shown to be temperature dependent on approaching the main (thermotropic) phase transition temperature T-m. In the case of DMPC/cholesterol bilayers, we also obtained evidence for a relation between the bending elasticity and the corresponding temperature/cholesterol molecular ratio phase diagram. Comparison of DMPC/cholesterol with DMPC/cholesterol sulfate bilayers at 30 degrees C containing 30% sterol ratio shows that k(c) is independent of the surface charge density of the bilayer, Finally, bending elasticities of red blood cell (RBC) total lipid extracts lead to a very low k(c) at 37 degrees C if we refer to DMPC/cholesterol bilayers. At 25 degrees C theory low bending elasticity of a cholesterol-free RBC lipid extract seems to be related to a phase coexistence, as it can be observed by solid-stale P-31-NMR. At the same temperature, the cholesterol-containing RBC lipid extract membrane shows an increase in the bending constant comparable to the one observed for a high cholesterol ratio in DMPC membranes.
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收藏
页码:2616 / 2629
页数:14
相关论文
共 77 条
[21]   GIANT VESICLE BILAYERS COMPOSED OF MIXTURES OF LIPIDS, CHOLESTEROL AND POLYPEPTIDES - THERMOMECHANICAL AND (MUTUAL) ADHERENCE PROPERTIES [J].
EVANS, E ;
NEEDHAM, D .
FARADAY DISCUSSIONS, 1986, 81 :267-280
[22]   PHYSICAL-PROPERTIES OF SURFACTANT BILAYER-MEMBRANES - THERMAL TRANSITIONS, ELASTICITY, RIGIDITY, COHESION, AND COLLOIDAL INTERACTIONS [J].
EVANS, E ;
NEEDHAM, D .
JOURNAL OF PHYSICAL CHEMISTRY, 1987, 91 (16) :4219-4228
[23]   BENDING ELASTICITY AND THERMAL FLUCTUATIONS OF LIPID-MEMBRANES - THEORETICAL AND EXPERIMENTAL REQUIREMENTS [J].
FAUCON, JF ;
MITOV, MD ;
MELEARD, P ;
BIVAS, I ;
BOTHOREL, P .
JOURNAL DE PHYSIQUE, 1989, 50 (17) :2389-2414
[24]   Comparative effects of cholesterol and cholesterol sulfate on hydration and ordering of dimyristoylphosphatidylcholine membranes [J].
Faure, C ;
Tranchant, JF ;
Dufourc, EJ .
BIOPHYSICAL JOURNAL, 1996, 70 (03) :1380-1390
[25]   TEMPERATURE AND CHAIN-LENGTH EFFECTS ON BENDING ELASTICITY OF PHOSPHATIDYLCHOLINE BILAYERS [J].
FERNANDEZPUENTE, L ;
BIVAS, I ;
MITOV, MD ;
MELEARD, P .
EUROPHYSICS LETTERS, 1994, 28 (03) :181-186
[26]  
FERNANDEZPUENTE L, 1994, THESIS U BORDEAUX 1
[27]   INTERACTION OF SHORT-CHAIN LECITHIN WITH LONG-CHAIN PHOSPHOLIPIDS - CHARACTERIZATION OF VESICLES THAT FORM SPONTANEOUSLY [J].
GABRIEL, NE ;
ROBERTS, MF .
BIOCHEMISTRY, 1986, 25 (10) :2812-2821
[28]   LATERAL AND TRANSVERSAL DIFFUSION AND PHASE-TRANSITIONS IN ERYTHROCYTE-MEMBRANES - EXCIMER FLUORESCENCE STUDY [J].
GALLA, HJ ;
LUISETTI, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 596 (01) :108-117
[29]   NEARLY SPHERICAL VESICLE SHAPES CALCULATED BY USE OF SPHERICAL-HARMONICS - AXISYMMETRICAL AND NONAXISYMMETRIC SHAPES AND THEIR STABILITY [J].
HEINRICH, V ;
BRUMEN, M ;
HEINRICH, R ;
SVETINA, S ;
ZEKS, B .
JOURNAL DE PHYSIQUE II, 1992, 2 (05) :1081-1108
[30]   ELASTIC PROPERTIES OF LIPID BILAYERS - THEORY AND POSSIBLE EXPERIMENTS [J].
HELFRICH, W .
ZEITSCHRIFT FUR NATURFORSCHUNG C-A JOURNAL OF BIOSCIENCES, 1973, C 28 (11-1) :693-703