Drosophila MSL complex globally acetylates H4K16 on the male X chromosome for dosage compensation

被引：108

作者：

Gelbart, Marnie E. ^{[1
,2
]}

Larschan, Erica ^{[1
,2
]}

Peng, Shouyong ^{[1
,2
,3
]}

Park, Peter J. ^{[1
,3
]}

Kuroda, Mitzi I. ^{[1
,2
]}

机构：

[1] Brigham & Womens Hosp, Dept Med, Div Genet, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA USA

[3] Childrens Hosp, Childrens Hosp Informat Program, Boston, MA 02115 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2009年 / 16卷 / 08期

基金：

美国国家卫生研究院;

关键词：

HISTONE H4 ACETYLATION; GENOME-WIDE ANALYSIS; CHROMATIN-STRUCTURE; H4-K16; ACETYLATION; ANALYSIS REVEALS; GENE-EXPRESSION; LYSINE; 16; MOF; MELANOGASTER; ACETYLTRANSFERASE;

D O I：

10.1038/nsmb.1644

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The Drosophila melanogaster male-specific lethal (MSL) complex binds the single male X chromosome to upregulate gene expression to equal that from the two female X chromosomes. However, it has been puzzling that similar to 25% of transcribed genes on the X chromosome do not stably recruit MSL complex. Here we find that almost all active genes on the X chromosome are associated with robust H4 Lys16 acetylation (H4K16ac), the histone modification catalyzed by the MSL complex. The distribution of H4K16ac is much broader than that of the MSL complex, and our results favor the idea that chromosome-wide H4K16ac reflects transient association of the MSL complex, occurring through spreading or chromosomal looping. Our results parallel those of localized Polycomb repressive complex and its more broadly distributed chromatin mark, trimethylated histone H3 Lys27 (H3K27me3), suggesting a common principle for the establishment of active and silenced chromatin domains.

引用

页码：825 / U47

页数：9

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