Comparison of conventional and fast gas chromatography in human plasma fatty acid determination

被引：80

作者：

Bondia-Pons, I ^{[1
]}

Castellote, AI ^{[1
]}

López-Sabater, MC ^{[1
]}

机构：

[1] Univ Barcelona, Fac Farm, Dept Nutr & Bromatol, CeRTA, E-08028 Barcelona, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 809卷 / 02期

关键词：

fatty acids;

D O I：

10.1016/j.jchromb.2004.07.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fast gas chromatographic technique for the determination of fatty acids in human plasma was developed. Its validation and comparison to a conventional method are here reported. The fast method significantly reduced the time required for analysis by a factor of 5 (total time of 3.2 min) while maintaining a similar resolution. Reproducibility of qualitative and quantitative data was measured in both applications. The results demonstrated that the applied fast gas chromatography (GC) conditions do not affect the analytical quality of the assays, allowing a short analysis time. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：339 / 344

页数：6

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