Mapping metabolites from rough terrain: laser ablation electrospray ionization on non-flat samples

被引：32

作者：

Bartels, Benjamin ^{[1
]}

Kulkarni, Purva ^{[1
,2
]}

Danz, Norbert ^{[3
]}

Boecker, Sebastian ^{[2
]}

Saluz, Hans Peter ^{[4
]}

Svatos, Ales ^{[1
]}

机构：

[1] Max Planck Inst Chem Ecol, Res Grp Mass Spectrometry Prote, Hans Knoll Str 8, D-07745 Jena, Germany

[2] Friedrich Schiller Univ Jena, Chair Bioinformat, Ernst Abbe Pl 2, D-07743 Jena, Germany

[3] Fraunhofer Inst Appl Opt & Precis Engn IOF, Albert Einstein Str 7, D-07745 Jena, Germany

[4] Leibniz Inst Nat Prod Res & Infect Biol, Dept Cell & Mol Biol, Adolf Reichwein Str 23, D-07745 Jena, Germany

来源：

RSC ADVANCES | 2017年 / 7卷 / 15期

关键词：

IMAGING MASS-SPECTROMETRY; CLINICAL-RESEARCH; CONFOCAL SENSOR; PRESSURE; TISSUE;

D O I：

10.1039/c6ra26854d

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

Established laser-based ionization experiments require the surface of a sample to be as flat as possible to guarantee optimal laser focus. A laser ablation electrospray ionization ( LAESI) source was custom-built to accommodate the topography of non-flat sample surfaces. Employing a confocal distance sensor, a height profile of the surface in question was recorded prior to the actual ionization experiment. The robustness of the system was evaluated by the metabolic profiling of radish ( Raphanus sativus) leaves, chosen due to their pronounced surface features and known content of specialized metabolites. After the ionization experiments, light microscopy imaging was performed to evaluate ablation crater size and position. Reproducible ablation mark diameters of 69 +/- 7 mu m in average have been achieved. Mass spectrometric imaging capability has been proven on R. sativus leaf samples as well.

引用

页码：9045 / 9050

页数：6

共 23 条

[1]

Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI [J].