Quantitation of caffeine by capillary zone electrophoresis with end-column amperometric detection at a carbon microdisk array electrode

被引：22

作者：

Jin, WR ^{[1
]}

Yu, DQ ^{[1
]}

Dong, Q ^{[1
]}

Ye, XY ^{[1
]}

机构：

[1] Shandong Univ, Sch Chem, Sci Analyt Lab, Jinan 250100, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2000年 / 38卷 / 01期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1093/chromsci/38.1.11

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary zone electrophoresis is employed for the determination of caffeine using end-column amperometric detection with a carbon fiber microdisk array electrode at a constant potential of 1.45 V versus a saturated calomel electrode. The optimum conditions of separation and detection are 0.152mM NaH2PO4-0.648mM Na2HPO4 for the buffer solution, 20 kV for the separation voltage, 5 kV for the injection voltage, and 10 s for the injection time. The limit of detection is 2.9 x 10-4mM or 1.2 fmol (signal-to-noise ratio = 2). The relative standard deviation is 0.68% for the migration time and 2.3% for the electrophoretic peak current. The method is applied to determining caffeine in human serum and a cola drink.

引用

页码：11 / 15

页数：5

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