Dynamics of Gene Silencing in a Live Cell: Stochastic Resonance

被引：15

作者：

Chattoraj, Shyamtanu ^{[1
]}

Saha, Shekhar ^{[2
]}

Jana, Siddhartha Sankar ^{[2
]}

Bhattacharyya, Kankan ^{[1
]}

机构：

[1] Indian Assoc Cultivat Sci, Dept Phys Chem, Kolkata 700032, India

[2] Indian Assoc Cultivat Sci, Dept Biol Chem, Kolkata 700032, India

来源：

JOURNAL OF PHYSICAL CHEMISTRY LETTERS | 2014年 / 5卷 / 06期

关键词：

NOISE; OSCILLATIONS; ENHANCEMENT; INFORMATION;

D O I：

10.1021/jz500152m

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Binding of a specific siRNA to the target mRNA in a live cell (human breast cancer cell, MCF-7) is studied by confocal microscopy. The specific siRNA (labeled with a fluorophore, alexa 488) exhibits much higher intensity of fluorescence in the bound state than in the free (unbound) state. It is observed that repeated unbinding and rebinding of siRNA (to target mRNA) occur before gene silencing. 16 273 on-time periods (residence or dwell time of siRNA in bound form) are detected. They follow a strikingly simple pattern. All of the on-time periods are odd-integral multiples of 5.5 +/- 0.05 ms. This is ascribed to stochastic resonance.

引用

页码：1012 / 1016

页数：5

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