Picking a winner: new mechanistic insights into the design of effective siRNAs

被引：28

作者：

Gong, DQ

Ferrell, JE ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Mol Pharmacol, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2004年 / 22卷 / 09期

关键词：

D O I：

10.1016/j.tibtech.2004.07.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recent work has shown that the efficacy of a small interfering RNA [siRNA) for silencing gene expression is a function of how easy it is to unwind the siRNA from the 5'-antisense end. Based on these insights, one group has designed an algorithm that substantially improves the odds of picking an effective siRNA, and two groups have shown that 'forked' or 'frayed' siRNAs, which should be easier to unwind from the W-antisense end, are more effective than conventional siRNAs. These strategies represent important steps towards the rational design of effective siRNAs.

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收藏

页码：451 / 454

页数：4

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