A PCR-based method for detection and quantification of small RNAs

被引：136

作者：

Ro, Seungil ^{[1
]}

Park, Chanjae ^{[1
]}

Jin, Jingling ^{[1
]}

Sanders, Kenton M. ^{[1
]}

Yan, Wei ^{[1
]}

机构：

[1] Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89557 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 351卷 / 03期

关键词：

micro RNA (miRNA); Piwi-interacting RNA (piRNA); small nucleolar RNAs (snoRNAs); real-time quantitative PCR (Q-PCR); small RNA cDNA (srcDNA);

D O I：

10.1016/j.bbrc.2006.10.105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：756 / 763

页数：8

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