Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling

被引:43
作者
Christensen, Trine [1 ]
Trabbic-Carlson, Kimberly [1 ]
Liu, Wenge [1 ]
Chilkoti, Ashutosh [1 ]
机构
[1] Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA
关键词
D O I
10.1016/j.ab.2006.09.020
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
[No abstract available]
引用
收藏
页码:166 / 168
页数:3
相关论文
共 17 条
[1]   Simple bioseparations using self-cleaving elastin-like polypeptide tags [J].
Banki, MR ;
Feng, LA ;
Wood, DW .
NATURE METHODS, 2005, 2 (09) :659-661
[2]   Self-cleavable stimulus responsive tags for protein purification without chromatography [J].
Ge, X ;
Yang, DSC ;
Trabbic-Carlson, K ;
Kim, B ;
Chilkoti, A ;
Filipe, CDM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (32) :11228-11229
[3]  
HOLMGREN A, 1984, METHOD ENZYMOL, V107, P295
[4]   Temperature-triggered purification of antibodies [J].
Kim, JY ;
Mulchandani, A ;
Chen, W .
BIOTECHNOLOGY AND BIOENGINEERING, 2005, 90 (03) :373-379
[5]   Comparison of affinity tags for protein purification [J].
Lichty, JJ ;
Malecki, JL ;
Agnew, HD ;
Michelson-Horowitz, DJ ;
Tan, S .
PROTEIN EXPRESSION AND PURIFICATION, 2005, 41 (01) :98-105
[6]   Product purification by reversible phase transition following Escherichia coli expression of genes encoding up to 251 repeats of the elastomeric pentapeptide GVGVP [J].
McPherson, DT ;
Xu, J ;
Urry, DW .
PROTEIN EXPRESSION AND PURIFICATION, 1996, 7 (01) :51-57
[7]   Protein purification by fusion with an environmentally responsive elastin-like polypeptide: Effect of polypeptide length on the purification of thioredoxin [J].
Meyer, DE ;
Trabbic-Carlson, K ;
Chilkoti, A .
BIOTECHNOLOGY PROGRESS, 2001, 17 (04) :720-728
[8]   Genetically encoded synthesis of protein-based polymers with precisely specified molecular weight and sequence by recursive directional ligation: Examples from the elastin-like polypeptide system [J].
Meyer, DE ;
Chilkoti, A .
BIOMACROMOLECULES, 2002, 3 (02) :357-367
[9]   Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides [J].
Meyer, DE ;
Chilkoti, A .
BIOMACROMOLECULES, 2004, 5 (03) :846-851
[10]   Purification of recombinant proteins by fusion with thermally-responsive polypeptides [J].
Meyer, DE ;
Chilkoti, A .
NATURE BIOTECHNOLOGY, 1999, 17 (11) :1112-1115