Micromechanical detection of proteins using aptamer-based receptor molecules

被引:236
作者
Savran, CA
Knudsen, SM
Ellington, AD
Manalis, SR
机构
[1] MIT, Media Lab, Div Biol Engn, Cambridge, MA 02139 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
关键词
D O I
10.1021/ac049859f
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We report label-free protein detection using a microfabricated cantilever-based sensor that is functionalized with DNA aptamers to act as receptor molecules. The sensor utilizes two adjacent cantilevers that constitute a sensor/reference pair and allows direct detection of the differential bending between the two cantilevers. One cantilever is functionalized with aptamers selected for Taq DNA polymerase while the other is blocked with single-stranded DNA. We have found that the polymerase-aptamer binding induces a change in surface stress, which causes a differential cantilever bending that ranges from 3 to 32 nm depending on the ligand concentration. Protein recognition on the sensor surface is specific and has a concentration dependence that is similar to that in solution.
引用
收藏
页码:3194 / 3198
页数:5
相关论文
共 17 条
  • [1] Label-free protein assay based on a nanomechanical cantilever array
    Arntz, Y
    Seelig, JD
    Lang, HP
    Zhang, J
    Hunziker, P
    Ramseyer, JP
    Meyer, E
    Hegner, M
    Gerber, C
    [J]. NANOTECHNOLOGY, 2003, 14 (01) : 86 - 90
  • [2] A FEMTOJOULE CALORIMETER USING MICROMECHANICAL SENSORS
    BARNES, JR
    STEPHENSON, RJ
    WOODBURN, CN
    OSHEA, SJ
    WELLAND, ME
    RAYMENT, T
    GIMZEWSKI, JK
    GERBER, C
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 1994, 65 (12) : 3793 - 3798
  • [3] Automated selection of aptamers against protein targets translated in vitro:: from gene to aptamer -: art. no. e108
    Cox, JC
    Hayhurst, A
    Hesselberth, J
    Bayer, TS
    Georgiou, G
    Ellington, AD
    [J]. NUCLEIC ACIDS RESEARCH, 2002, 30 (20) : e108
  • [4] Translating biomolecular recognition into nanomechanics
    Fritz, J
    Baller, MK
    Lang, HP
    Rothuizen, H
    Vettiger, P
    Meyer, E
    Güntherodt, HJ
    Gerber, C
    Gimzewski, JK
    [J]. SCIENCE, 2000, 288 (5464) : 316 - 318
  • [5] The RNA aptamer-binding site of hepatitis C virus NS3 protease
    Hwang, J
    Fauzi, H
    Fukuda, K
    Sekiya, S
    Kakiuchi, N
    Shimotohno, K
    Taira, K
    Kusakabe, I
    Nishikawa, S
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 279 (02) : 557 - 562
  • [6] In vitro selection of signaling aptamers
    Jhaveri, S
    Rajendran, M
    Ellington, AD
    [J]. NATURE BIOTECHNOLOGY, 2000, 18 (12) : 1293 - 1297
  • [7] Optimization and performance of high-resolution micro-optomechanical thermal sensors
    Lai, J
    Perazzo, T
    Shi, Z
    Majumdar, A
    [J]. SENSORS AND ACTUATORS A-PHYSICAL, 1997, 58 (02) : 113 - 119
  • [8] A fiber-optic microarray biosensor using aptamers as receptors
    Lee, M
    Walt, DR
    [J]. ANALYTICAL BIOCHEMISTRY, 2000, 282 (01) : 142 - 146
  • [9] Inhibition of multiple thermostable DNA polymerases by a heterodimeric aptamer
    Lin, Y
    Jayasena, SD
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1997, 271 (01) : 100 - 111
  • [10] An aptamer-based quartz crystal protein biosensor
    Liss, M
    Petersen, B
    Wolf, H
    Prohaska, E
    [J]. ANALYTICAL CHEMISTRY, 2002, 74 (17) : 4488 - 4495