Micromechanical detection of proteins using aptamer-based receptor molecules

被引:236
作者
Savran, CA
Knudsen, SM
Ellington, AD
Manalis, SR
机构
[1] MIT, Media Lab, Div Biol Engn, Cambridge, MA 02139 USA
[2] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
关键词
D O I
10.1021/ac049859f
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We report label-free protein detection using a microfabricated cantilever-based sensor that is functionalized with DNA aptamers to act as receptor molecules. The sensor utilizes two adjacent cantilevers that constitute a sensor/reference pair and allows direct detection of the differential bending between the two cantilevers. One cantilever is functionalized with aptamers selected for Taq DNA polymerase while the other is blocked with single-stranded DNA. We have found that the polymerase-aptamer binding induces a change in surface stress, which causes a differential cantilever bending that ranges from 3 to 32 nm depending on the ligand concentration. Protein recognition on the sensor surface is specific and has a concentration dependence that is similar to that in solution.
引用
收藏
页码:3194 / 3198
页数:5
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