Characterization of fluorescent phospholipid liposomes entrapped in sol-gel derived silica

被引:35
作者
Besanger, T [1 ]
Zhang, Y [1 ]
Brennan, JD [1 ]
机构
[1] McMaster Univ, Dept Chem, Hamilton, ON L8S 4M1, Canada
关键词
D O I
10.1021/jp0263525
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Bilayer lipid membranes (BLMs) have been widely examined as sensing elements for a variety of analytes, in. both the vapor and solution phases, using electrochemical, acoustic wave, and fluorescence methods. For successful development of stable sensing devices, it is necessary to be able to immobilize the BLMs in a manner that allows long-term retention of the membrane structure and still permits large-scale structural reorganizations such as phase transitions. In this work, small unilamellar liposomes were formed from either 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or L-a-phosphatidylcholine (egg PC) and were doped with 1-5 mol % of the fluorescent probes diphenylhexatriene (DPH) or nitrobenzoxadiazole-labeled dipalmitoylphosphatidylethanolamine (NBD-PE). The liposomes were entrapped in a series of different sol-gel derived silicate materials and the stability and phase-transition behavior of the liposomes was characterized. DPPC was observed to undergo reversible phase transitions when entrapped in glasses derived from either sodium silicate or a diglyceryl silane precursor; however, liposomes did not undergo phase transitions when entrapped in tetraethyl orthosilicate derived glasses, indicating that they had likely ruptured during the encapsulation process. As a practical demonstration of the use of the immobilized membranes for sensing applications, we have examined the use of pH-induced phase transitions as a means of generating a fluorescence signal that is based on changes in self-quenching of NBD-PE within liposomes composed of DPPC and dipalmitoylphosphatidic acid (DPPA). The results show that such. pH-induced phase transitions occur for the entrapped vesicles and that the fluorescence responses follow the pH dependence of DPPA.
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页码:10535 / 10542
页数:8
相关论文
共 73 条
[51]  
OConnor D., 2012, Time-Correlated Single Photon Counting
[52]   FORMATION OF PLANAR SOLVENT-FREE PHOSPHOLIPID-BILAYERS BY LANGMUIR-BLODGETT TRANSFER OF MONOLAYERS TO MICROMACHINED APERTURES IN SILICON [J].
OSBORN, TD ;
YAGER, P .
LANGMUIR, 1995, 11 (01) :8-12
[53]  
PARASASSI T, 1984, J BIOL CHEM, V259, P4011
[54]   Amperometric detection of Escherichia coli heat labile enterotoxin by redox diacetylenic vesicles on a sol-gel thin film electrode [J].
Peng, TZ ;
Chen, Q ;
Stevens, RC .
ANALYTICAL CHEMISTRY, 2000, 72 (07) :1611-1617
[55]  
PERCOT A, 2000, BIOCONG CHEM, V11, P647
[56]  
SASAKI DY, 1999, P SPIE INT SOC OPT E, V3062, P275
[57]  
SCHUSTER B, 2000, REV MOL BIOTECHNOL, V74, P2
[58]   SEPARATION PROCEDURES USED TO REVEAL AND FOLLOW DRUG PROTEIN-BINDING [J].
SEBILLE, B ;
ZINI, R ;
MADJAR, CV ;
THUAUD, N ;
TILLEMENT, JP .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 531 :51-77
[59]   Glucose minisensor based on self-assembled biotinylated phospholipid membrane on a solid support and its physical properties [J].
Snejdarkova, M ;
Rehak, M ;
Babincova, M ;
Sargent, DF ;
Hianik, T .
BIOELECTROCHEMISTRY AND BIOENERGETICS, 1997, 42 (01) :35-42
[60]   DESIGN OF A GLUCOSE MINISENSOR BASED ON STREPTAVIDIN GLUCOSE-OXIDASE COMPLEX COUPLING WITH SELF-ASSEMBLED BIOTINYLATED PHOSPHOLIPID MEMBRANE ON SOLID SUPPORT [J].
SNEJDARKOVA, M ;
REHAK, M ;
OTTO, M .
ANALYTICAL CHEMISTRY, 1993, 65 (06) :665-668