Color fingerprinting of proteins by calixarenes embedded in lipid/polydiacetylene vesicles

被引：116

作者：

Kolusheva, Sofiya

Zadmard, Reza

Schrader, Thomas

Jelinek, Raz ^{[1
]}

机构：

[1] Univ Marburg, Dept Chem, D-35032 Marburg, Germany

[2] Ben Gurion Univ Negev, Ilse Katz Ctr Nanotechnol, IL-84105 Beer Sheva, Israel

[3] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 41期

关键词：

D O I：

10.1021/ja064957z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Naked eye color detection of proteins was achieved by embedding calixarene receptors within vesicles comprising phospholipids and the chromatic polymer polydiacetylene. Dramatic visible absorbance changes were induced through electrostatic interactions between the protein surface and the vesicle-incorporated hosts. The colorimetric responses could be induced by micromolar protein concentrations, and furthermore, specific protein fingerprints could be obtained by incorporating different receptors within the vesicles. Fluorescence and circular dichroism experiments confirmed the relationship between the colorimetric phenomena and protein docking on the surface of the chromatic vesicles. The colorimetric assay constitutes a generic platform for high-sensitivity detection of soluble proteins and for evaluation of protein surface charge distribution.

引用

页码：13592 / 13598

页数：7

共 30 条

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