Antibody manufacture in transgenic animals and comparisons with other systems

被引:56
作者
Houdebine, LM [1 ]
机构
[1] INRA, F-78352 Jouy En Josas, France
关键词
D O I
10.1016/S0958-1669(02)00362-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Various forms of recombinant monoclonal antibodies are being used increasingly, mainly for therapeutic purposes. The isolation and engineering of the corresponding genes is becoming less of a bottleneck in the process; however, the production of recombinant antibodies is itself a limiting factor and a shortage is expected in the coming years. Milk from transgenic animals appears to be one of the most attractive sources of recombinant antibodies. None of the production systems presently implemented (CHO cells, insect cells infected by baculovirus, or transgenic animals and plants) has yet been optimized. This review describes the advantages of using milk for antibody production in comparison with the other systems.
引用
收藏
页码:625 / 629
页数:5
相关论文
共 59 条
  • [1] Recombinant protein expression for therapeutic applications
    Andersen, DC
    Krummen, L
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (02) : 117 - 123
  • [2] ANDERSSON R, 2001, BUSINESS MED REPORT
  • [3] The effect of a monoclonal antibody coupled to ricin A chain-derived peptides on endothelial cells in vitro:: Insights into toxin-mediated vascular damage
    Baluna, R
    Coleman, E
    Jones, C
    Ghetie, V
    Vitetta, ES
    [J]. EXPERIMENTAL CELL RESEARCH, 2000, 258 (02) : 417 - 424
  • [4] In vitro comparison of the antigen-binding and stability properties of the various molecular forms of IgA antibodies assembled and produced in CHO cells
    Berdoz, J
    Blanc, CT
    Reinhardt, M
    Kraehenbuhl, JP
    Corthésy, B
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (06) : 3029 - 3034
  • [5] Human anti-rhesus D IgG1 antibody produced in transgenic plants
    Bouquin, T
    Thomsen, M
    Nielsen, LK
    Green, TH
    Mundy, J
    Dziegiel, MH
    [J]. TRANSGENIC RESEARCH, 2002, 11 (02) : 115 - 122
  • [6] Antibodies for defense against biological attack
    Casadevall, A
    [J]. NATURE BIOTECHNOLOGY, 2002, 20 (02) : 114 - 114
  • [7] Antibody mediated protection against intracellular pathogens
    Casadevall, A
    [J]. TRENDS IN MICROBIOLOGY, 1998, 6 (03) : 102 - 107
  • [8] Engineering passive immunity in transgenic mice secreting virus-neutralizing antibodies in milk
    Castilla, J
    Pintado, B
    Sola, I
    Sánchez-Morgado, JM
    Enjuanes, L
    [J]. NATURE BIOTECHNOLOGY, 1998, 16 (04) : 349 - 354
  • [9] Therapeutic antibody expression technology
    Chadd, HE
    Chamow, SM
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (02) : 188 - 194
  • [10] A murine monoclonal antibody produced in transgenic plants with plant-specific glycans is not immunogenic in mice
    Chargelegue, D
    Vine, ND
    van Dolleweerd, CJ
    Drake, PMW
    Ma, JKC
    [J]. TRANSGENIC RESEARCH, 2000, 9 (03) : 187 - 194