Selective transcriptional regulations in the human liver cell by hepatitis B viral X protein

被引：56

作者：

Han, JS ^{[1
]}

Yoo, HY ^{[1
]}

Choi, BH ^{[1
]}

Rho, HM ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Biol Sci, Seoul 151742, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2000年 / 272卷 / 02期

关键词：

HBx; cDNA microarray; selective transcriptional regulation;

D O I：

10.1006/bbrc.2000.2801

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hepatitis B viral X protein (HBx) is known as a transcription factor and potential oncogene. To gain a better view of the effect of HBx on the transcriptional regulation in the human liver cell, we constructed a HepG2 cell line stably expressing HBx (HepG2-HBx), and performed cDNA microarray analysis on 588 cellular cDNAs comparing with untransformed control cells. Two genes (IGFR-2, RhoA) of oncogenes, one gene (p55CDC) of cell cycle regulators, three genes (thrombin receptor, MLK-3, MacMARCKS) of intracellular transducers, one gene (HSP27) of stress response proteins, two genes (FAST kinase, Bak) of apoptosis response proteins, one gene (p21(WAF)) Of transcription factors were highly up-regulated; one gene (transcription elongation factor SII) of transcription factors and two genes (monocyte chemotactic protein 1, T-lymphocyte-secreted protein I-309) of growth factors were highly down-regulated. These results showed selective transcriptional regulation by HBx in the human liver cell. (C) 2000 Academic Press.

引用

页码：525 / 530

页数：6

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