Presence and release of SR-17 (chromogranin B586-602) in the porcine splenic nerve and its enzymatic degradation by CD26/dipeptidyl peptidase IV

被引：6

作者：

Depreitere, J

Durinx, C

Wang, ZS

Coen, E

Lambeir, AM

Scharpé, S

De Potter, W

Nouwen, EJ

机构：

[1] Univ Antwerp, UIA, Lab Neurobiol & Neuropharmacol, B-2610 Wilrijk, Antwerp, Belgium

[2] Univ Antwerp, UIA, Med Biochem Lab, B-2610 Wilrijk, Antwerp, Belgium

来源：

REGULATORY PEPTIDES | 2002年 / 106卷 / 1-3期

关键词：

secretogranin I; CgB; processing; sympathetic nerve; DPP IV;

D O I：

10.1016/S0167-0115(02)00038-1

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Using the pig splenic nerve as a model, we investigated the proteolytic processing of porcine chromogranin B (CgB) during its axonal transport. An ELISA was developed for SR-17 (CgB(586-602)), a novel CgB-derived peptide, originally found in the adrenal medulla. The results demonstrate that CgB is processed in an early stage during its axonal transport. Immunohistochemical data, based on a rabbit anti-SR-17 antiserum, show that the spleen CgB/SR-17 is exclusively present in the nerve endings. No SR-17 immunoreactivity (IR) was found in splenocytes. We also provide evidence that SR-17 is co-released with noradrenaline (NA) upon electrical stimulation of the splenic nerve. Its release is frequency-dependent and strongly enhanced in the presence of the alpha-blocking agent phentolamine. In addition, we show that the new CgB-peptide can serve as a substrate for the lymphocyte surface glycoprotein CD26, also known as dipeptidyl peptidase IV (DPP IV), generating a new peptide ER-15 (CgB(588-602)). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：71 / 79

页数：9

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