Recent advancements in surface-enhanced laser desorption/ionization-time of flight-mass spectrometry

被引：79

作者：

Merchant, M ^{[1
]}

Weinberger, SR ^{[1
]}

机构：

[1] Ciphergen Biosyst Inc, Palo Alto, CA 94306 USA

来源：

ELECTROPHORESIS | 2000年 / 21卷 / 06期

关键词：

surface-enhanced laser desorption; ionization; review;

D O I：

10.1002/(SICI)1522-2683(20000401)21:6<1164::AID-ELPS1164>3.3.CO;2-S

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The overall history and recent advances in surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) technology is reviewed herein. Fundamentals of SELDI-TOF analysis are presented while drawing comparisons with other laser-based mass spectrometry techniques. The application of SELDI-TOF-MS to functional genomics and biomarker discovery is discussed and exemplified by elucidating a biomarker candidate for prostatic carcinoma. Finally, a short discussion regarding future SELDI requirements and developments is supplied.

引用

页码：1164 / 1177

页数：14

共 85 条

[81] DIRECT MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRIC ANALYSIS OF PROTEINS IMMOBILIZED ON NYLON-BASED MEMBRANES [J].