Cloning and functional expression of a mungbean defensin VrD1 in Pichia pastoris

被引：42

作者：

Chen, JJ

Chen, GH

Hsu, HC

Li, SS

Chen, CS ^{[1
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Agr Chem, Taipei 106, Taiwan

[2] Natl Taiwan Ocean Univ, Grad Inst Biosic & Biotechnol, Chilung 202, Taiwan

[3] Acad Sinica, Inst Bot, Taipei 115, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2004年 / 52卷 / 08期

关键词：

defensin; insecticidal activity; mungbean; Pichia pastoris; protein synthesis;

D O I：

10.1021/jf030662i

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

It was shown previously that a bacterially expressed mungbean defensin VrCRP exhibited both antifungal and insecticidal activities. To isolate this protein in a large quantity for its characterization, the defensin cDNA was expressed in Pichia pastoris and the recombinant defensin (rVrD1) was purified. The recombinant VrD1 was shown to inhibit the growth of fungi such as Fusarium oxysporum, Pyricularia oryza, Rhizoctonia solani, and Trichophyton rubrum and development of bruchid larva. The protein also inhibits in vitro protein synthesis. These biological activities are similar to that of the bacterially expressed defensin. Functional expression of VrD1 in Pichia pastoris provides a highly feasible system to study the structure-function relationship of VrD1 using the mutagenesis approach.

引用

页码：2256 / 2261

页数：6

共 25 条

[1] cDNA cloning and heterologous expression of functional cysteine-rich antifungal protein Psd1 in the yeast Pichia pastoris [J].