Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties

被引：45

作者：

Zhou, Peng

Dragulescu-Andrasi, Anca

Bhattacharya, Birendra

O'Keefe, Heather

Vatta, Paolo

Hyldig-Nielsen, Jens J.

Ly, Danith H.

机构：

[1] Carnegie Mellon Univ, Dept Chem, Pittsburgh, PA 15213 USA

[2] Appl Biosyst Inc, Foster City, CA 94404 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2006年 / 16卷 / 18期

关键词：

cell-permeable; guanidine-based peptide nucleic acid; GPNA; monomers; oligomers; synthesis; endoplasmic reticulum; ER;

D O I：

10.1016/j.bmcl.2006.06.052

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Guanidine-based peptide nucleic acid (GPNA) monomers and oligomers containing all four natural (adenine (A), cytosine (C), guanine (G), and thymine (T)) and two unnatural (2-thiouracil (U-s) and 2,6-diaminopurine (D)) nucleobases have been synthesized. Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when incubated with mammalian cells, localized specifically to the endoplasmic reticulum (ER). (c) 2006 Elsevier Ltd. All rights reserved.

引用

收藏

页码：4931 / 4935

页数：5

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