Investigation of microfabrication of biological sample arrays using piezoelectric and bubble-jet printing technologies

被引:59
作者
Allain, LR
Stratis-Cullum, DN
Vo-Dinh, T
机构
[1] Oak Ridge Natl Lab, Div Life Sci, Adv Biomed Sci, Oak Ridge, TN 37831 USA
[2] Oak Ridge Natl Lab, Div Life Sci, Technol Grp, Oak Ridge, TN 37831 USA
[3] Merck & Co Inc, Pharmaceut Anal & Control, W Point, PA 19486 USA
[4] USA, Res Lab, AMSRL SE EO, Adelphi, MD 20783 USA
关键词
ink-jet; bubble-jet; spotting; array; DNA; microarray; Bacillus anthracis; hybridization;
D O I
10.1016/j.aca.2004.04.065
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Modified commercial ink-jet printers are simple and cost-effective tools for the fabrication of microarrays containing biological samples. In this paper, the two most common ink-dispensing technologies, thermal and piezoelectric, were investigated, tested, and compared in terms of their ease of use and spotting quality. Ink cartridges were used as sample reservoirs and refilled with small volumes (as low as 20 muL) of water-based sample solutions containing herring sperm DNA and fluorescent dyes. Different solution compositions were evaluated and compared. Microscopic spots ( < 200 mum) were printed on various membranes (cellulose, nylon, nitrocellulose, and Zeta-Probe) and glass microscope slides. This technique enabled rapid preparation of a large number of printed arrays of biological materials at extremely low cost. To further demonstrate the utility of the microarray platform, we performed an assay of Bacillus anthracis, detected through DNA hybridization using fluorescent probes complementary to the B. anthracis target printed on a membrane. A comparison of Zeta-Probe and nylon membranes yielded less non-specific binding when using the Zeta-Probe membrane. In addition, detection of 100 fmol of the target per printed spot was demonstrated. (C) 2004 Published by Elsevier B.V.
引用
收藏
页码:77 / 85
页数:9
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