BlastDigester - a web-based program for efficient CAPS marker design

被引：20

作者：

Ilic, K ^{[1
]}

Berleth, T ^{[1
]}

Provart, NJ ^{[1
]}

机构：

[1] Univ Toronto, Dept Bot, Toronto, ON M5S 3B2, Canada

来源：

TRENDS IN GENETICS | 2004年 / 20卷 / 07期

关键词：

D O I：

10.1016/j.tig.2004.04.012

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Single nucleotide polymorphisms (SNPs) and insertion and deletions (InDels), the most common type of intra-specific sequence polymorphism, are rich resources for creating DNA markers for genotyping. In this article, we present a web-based program for generating cleaved amplified polymorphic sequence (CAPS) markers, PCR-based markers that use polymorphism in restriction endonuclease digestion patterns (snip-SNPs). Blast-Digester computationally 'digests' the sequence alignments that are returned in a Blast search, identifies snip-SNPs and simplifies PCR-primer generation through dynamic links to Primer3, a primer design program.

引用

页码：280 / 283

页数：4

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