DNA hybridization detection on electrical microarrays using coulostatic pulse technique

被引:21
作者
Dharuman, V.
Nebling, E.
Grunwald, T.
Albers, J.
Blohm, L.
Elsholz, B.
Woerl, R.
Hintsche, R.
机构
[1] Fraunhofer Inst Silicon Technol, Dept Biotech Microsyst, D-25524 Itzehoe, Germany
[2] Ebiochip Syst GmbH, Dept Biotech Microsyst, D-25524 Itzehoe, Germany
关键词
coulostatic pulse technique; DNA hybridization; label free detection; IDA electrodes; Si-technology; impedance; electrical array biochips;
D O I
10.1016/j.bios.2006.02.014
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
We demonstrated a novel application of transient coulostatic pulse technique for the detection of label free DNA hybridization on nm-sized gold interdigitated ultramicroelectrode arrays (Au-IDA) made in silicon technology. The array consists of eight different positions with an Au-IDA pair at each position arranged on the Si-based Biochip. Immobilization of capture probes onto the Au-IDA was accomplished by self-assembling of thiol-modified oligonucleotides. Target hybridization was indicated by a change in the magnitude of the time dependant potential relaxation curve in presence of electroactive Fe(CN)(6)(3-) in the phosphate buffer solution. While complementary DNA hybridization showed 50% increase in the relaxation potential, the non-complementary DNA showed a negligible change. A constant behaviour was noted for all positions. The dsDNA specific intercalating molecule, methylene blue, was found to be enhancing the discrimination effect. The changes in the relaxation potential curves were further corroborated following the ELISA like experiments using ExtraAvidine alkaline phosphatase labelling and redox recycling of para-aminophenol phosphate at IDAs. The coulostatic pulse technique was shown to be useful for identifying DNA sequences from brain tumour gene CK20, human herpes simplex virus, cytomegalovirus, Epstein-Barr virus and M13. phage. Compared to the hybridization of short chain ONTs (27 mers), the hybridization of long chain M 13 phage DNA showed three times higher increase in the relaxation curves. The method is fast enough to monitor hybridization interactions in milli or microsecond time scales and is well suitable for miniaturization and integration compared to the common impedance techniques for developing capacitative DNA sensors. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:744 / 751
页数:8
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