Clastosome:: A subtype of nuclear body enriched in 19S and 20S proteasomes, ubiquitin, and protein substrates of proteasome

被引:109
作者
Lafarga, M
Berciano, MT
Pena, E
Mayo, I
Castaño, JG
Bohmann, D
Rodrigues, JP
Tavanez, JP
Carmo-Fonseca, M [1 ]
机构
[1] Univ Lisbon, Fac Med, Inst Mol Med, P-1699 Lisbon, Portugal
[2] Univ Cantabria, Fac Med, Dept Anat & Cell Biol, Santander 39011, Spain
[3] Autonomous Univ Madrid, Fac Med, Dept Biochem, Madrid 28029, Spain
[4] Univ Rochester, Med Ctr, Ctr Canc Biol, Rochester, NY 14642 USA
关键词
D O I
10.1091/mbc.E02-03-0122
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Nuclear bodies represent a heterogeneous class of nuclear structures. Herein, we describe that a subset of nuclear bodies is highly enriched in components of the ubiquitin-proteasome pathway of proteolysis. We coined the term clastosome (from the Greek klastos, broken and soma, body) to refer to this type of nuclear body. Clastosomes contain a high concentration of 1) ubiquitin conjugates, 2) the proteolytically active 20S core and the 19S regulatory complexes of the 26S proteasome, and 3) protein substrates of the proteasome. Although detected in a variety of cell types, clastosomes are scarce under normal conditions; however, they become more abundant when proteasomal activity is stimulated. In contrast, clastosomes disappear when cells are treated with proteasome inhibitors. Protein substrates of the proteasome that are found concentrated in clastosomes include the short-lived transcription factors c-Fos and c-Jun, adenovirus E1A proteins, and the PML protein. We propose that clastosomes are sites where proteolysis of a variety of protein substrates is taking place.
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收藏
页码:2771 / 2782
页数:12
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