The MGF expression of osteoblasts in response to mechanical overload

被引：71

作者：

Tang, Li-Ling ^{[1
]}

Xian, Cheng-Yu ^{[1
]}

Wang, Yuan-Liang ^{[1
]}

机构：

[1] Chongqing Univ, Bioengn Coll, Key Lab Biomech & Tissue Engn, Minist Educ, Chongqing 40004, Peoples R China

来源：

ARCHIVES OF ORAL BIOLOGY | 2006年 / 51卷 / 12期

关键词：

osteoblast; stretch; IGF-I; MGF; proliferation;

D O I：

10.1016/j.archoralbio.2006.06.009

中图分类号：

R78 [口腔科学];

学科分类号：

1003 [口腔医学];

摘要：

Cell proliferation and mRNA expression of insulin-like growth factor (IGF-I) and 'mechanogrowth factor' (MGF) were studied in osteoblasts in response to overload. Static and cyctic-stretching were used to apply superphysiological strains to cells. Overload was found to increase cell growth. IGF-I and its splicing variant, MGF, were measured using reverse transcriptase-polymerase chain reaction method and were found to be regulated differentially by mechanical signals at the mRNA level. Cyctic-stretching had a more significant effect on cell proliferation and mRNA expression levels of IGF-I and MGF, white unstrained cells did not express MGF at the mRNA level. These results demonstrated that gene expression is regulated by mechanical stimulation. MGF expression in osteoblasts in response to strain may be related to an autocrine mechanism. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：1080 / 1085

页数：6

共 28 条

[1]

Different levels of neuroprotection by two insulin-like growth factor-I splice variants [J].