Development of a hypersensitive detection method for human parvovirus B19 DNA

被引：10

作者：

Sato, K

Matsuda, E

Kamisango, K

Iwasaki, H

Matsubara, S

Matsunaga, Y

机构：

[1] Chugai Pharmaceut Co Ltd, Cent Res Labs, Toshima Ku, Tokyo 1718545, Japan

[2] Natl Inst Infect Dis, Shinjuku Ku, Tokyo 1628640, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 03期

关键词：

D O I：

10.1128/JCM.38.3.1241-1243.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A new detection method for human parvovirus B19 DNA,vas established using PCR coupled with a hybridization protection assay. The amplified product was detected using acridinium ester-labeled DNA probes. By this method, a few copies of B19 DNA were detected in human serum albumin.

引用

页码：1241 / 1243

页数：3

共 22 条

[21] LARGE-SCALE SCREENING FOR HUMAN PARVOVIRUS B19 DNA IN CLINICAL SPECIMENS BY DOT-BLOT HYBRIDIZATION AND POLYMERASE CHAIN-REACTION [J].