Polydimethylsiloxane microfluidic chip with integrated microheater and thermal sensor

被引：78

作者：

Wu, Jinbo ^{[1
,2
]}

Cao, Wenbin ^{[3
]}

Wen, Weijia ^{[1
,2
]}

Chang, Donald Choy ^{[3
]}

Sheng, Ping ^{[1
,2
]}

机构：

[1] Hong Kong Univ Sci & Technol, Dept Phys, Kowloon, Hong Kong, Peoples R China

[2] Hong Kong Univ Sci & Technol, KAUST HKUST Micro Nanofluid Joint Lab, Kowloon, Hong Kong, Peoples R China

[3] Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China

来源：

BIOMICROFLUIDICS | 2009年 / 3卷 / 01期

关键词：

biochemistry; bioMEMS; biothermics; DNA; elastomers; enzymes; feedback; heating elements; lab-on-a-chip; microfluidics; microsensors; temperature control; temperature distribution; temperature sensors; POLYMERASE-CHAIN-REACTION; DNA AMPLIFICATION; GENETIC-ANALYSIS; PCR CHIP; DEVICES; ELECTROPHORESIS; MICROSYSTEM; CAPILLARY;

D O I：

10.1063/1.3058587

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A microheater and a thermal sensor were fabricated inside elastomeric polydimethylsiloxane microchannels by injecting silver paint (or other conductive materials) into the channels. With a high-precision control scheme, microheaters can be used for rapid heating, with precise temperature control and uniform thermal distribution. Using such a microheater and feedback system, a polymerase chain reaction experiment was carried out whereas the DNA was successfully amplified in 25 cycles, with 1 min per cycle.

引用

页数：7

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