Optically Modulated Fluorophores for Selective Fluorescence Signal Recovery

被引：115

作者：

Richards, Chris I.

Hsiang, Jung-Cheng

Senapati, Dulal

Patel, Sandeep

Yu, Junhua

Vosch, Tom

Dickson, Robert M. ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA

[2] Georgia Inst Technol, Petit Inst Biosci & Bioengn, Atlanta, GA 30332 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 13期

关键词：

LOCK-IN DETECTION; SINGLE-MOLECULE; LIVING CELLS; MICROSCOPY; DNA; SPECTROSCOPY; DYNAMICS;

D O I：

10.1021/ja809785s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Fluorescence imaging in biological sciences is hindered by significant depth-dependent signal attenuation and highly fluorescent backgrounds. We have developed optically modulated near-IR-emitting few-atom Ag nanodots that are selectively and dynamically photobrightened upon simultaneous excitation with a secondary laser, enabling high-sensitivity image extraction to reveal only the demodulated fluorophores. Image demodulation is demonstrated in high-background environments to extract weak signals from completely obscuring background emission. © 2009 American Chemical Society.

引用

收藏

页码：4619 / 4621

页数：3

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