Endophytic occupation of peanut root nodules by opportunistic Gammaproteobacteria

被引：94

作者：

Ibanez, Fernando ^{[1
]}

Angelini, Jorge ^{[1
]}

Taurian, Tania ^{[1
]}

Tonelli, Maria Laura ^{[1
]}

Fabra, Adriana ^{[1
]}

机构：

[1] Univ Nacl Rio Cuarto, Fac Ciencias Exactas Fis Quim & Nat, Dept Ciencias Nat, RA-5800 Rio Cuarto, Cordoba, Argentina

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 2009年 / 32卷 / 01期

关键词：

Arachis hypogaea; Bradyrhizobium; Pseudomonas; Klebsiella; Enterobacter; Endophytes from nodules; SP-NOV; LEGUMES; RHIZOBIA; GENE; SYMBIOSES; BACTERIA; STRAINS; DIVERSE; SOIL;

D O I：

10.1016/j.syapm.2008.10.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several bacterial isolates were recovered from surface-sterilized root nodules of Arachis hypogaea L. (peanut) plants growing in soils from Cordoba, Argentina. The 16S rDNA sequences of seven fast-growing strains were obtained and the phylogenetic analysis showed that these isolates belonged to the Phylum Proteobacteria, Class Gammaproteobacteria, and included Pseudomonas spp., Enterobacter spp., and Klebsiella spp. After storage, these strains became unable to induce nodule formation in Arachis hypogaea L. plants, but they enhanced plant yield. When the isolates were co-inoculated with an infective Bradyrhizobium strain, they were even found colonizing pre-formed nodules. Analysis of symbiotic genes showed that the nifH gene was only detected for the Klebsiella-like isolates and the nodC gene could not be amplified by PCR or be detected by Southern blotting in any of the isolates. The results obtained support the idea that these isolates are opportunistic bacteria able to colonize nodules induced by rhizobia. (C) 2008 Elsevier GmbH. All rights reserved.

引用

页码：49 / 55

页数：7

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