An Uncharacterized Apocarotenoid-Derived Signal Generated in ζ-Carotene Desaturase Mutants Regulates Leaf Development and the Expression of Chloroplast and Nuclear Genes in Arabidopsis

被引：158

作者：

Avendano-Vazquez, Aida-Odette ^{[1
]}

Cordoba, Elizabeth ^{[1
]}

Llamas, Ernesto ^{[1
]}

San Roman, Carolina ^{[1
]}

Nisar, Nazia ^{[2
]}

De la Torre, Susana ^{[1
]}

Ramos-Vega, Maricela ^{[1
]}

de la Luz Gutierrez-Nava, Maria ^{[1
]}

Cazzonelli, Christopher Ian ^{[3
]}

Pogson, Barry James ^{[2
]}

Leon, Patricia ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Mol Plantas, Cuernavaca 62210, Morelos, Mexico

[2] Australian Natl Univ, Res Sch Biol, Australian Res Council Ctr Excellence Plant Energ, Canberra, ACT 0200, Australia

[3] Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia

来源：

PLANT CELL | 2014年 / 26卷 / 06期

基金：

澳大利亚研究理事会;

关键词：

PHYTOENE DESATURASE; BETA-CAROTENE; PLANTS; BIOSYNTHESIS; ROOT; CELL; PATHWAY; PROTEIN; ENZYME; FAMILY;

D O I：

10.1105/tpc.114.123349

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In addition to acting as photoprotective compounds, carotenoids also serve as precursors in the biosynthesis of several phytohormones and proposed regulatory signals. Here, we report a signaling process derived from carotenoids that regulates early chloroplast and leaf development. Biosynthesis of the signal depends on zeta-carotene desaturase activity encoded by the zeta-CAROTENE DESATURASE (ZDS)/CHLOROPLAST BIOGENESIS5 (CLB5) gene in Arabidopsis thaliana. Unlike other carotenoid-deficient plants, zds/clb5 mutant alleles display profound alterations in leaf morphology and cellular differentiation as well as altered expression of many plastid-and nucleus-encoded genes. The leaf developmental phenotypes and gene expression alterations of zds/clb5/spc1/pde181 plants are rescued by inhibitors or mutations of phytoene desaturase, demonstrating that phytofluene and/or zeta-carotene are substrates for an unidentified signaling molecule. Our work further demonstrates that this signal is an apocarotenoid whose synthesis requires the activity of the carotenoid cleavage dioxygenase CCD4.

引用

页码：2524 / 2537

页数：14

共 73 条

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