Integration of a wet analysis system on a glass chip: determination of Co(II) as 2-nitroso-1-naphthol chelates by solvent extraction and thermal lens microscopy

被引:80
作者
Minagawa, T
Tokeshi, M
Kitamori, T
机构
[1] Univ Tokyo, Grad Sch Engn, Dept Appl Chem, Bunkyo Ku, Tokyo 1138656, Japan
[2] Kanagawa Acad Sci & Technol, Integrated Chem Project, Takatsu Ku, Kawasaki, Kanagawa, Japan
来源
LAB ON A CHIP | 2001年 / 1卷 / 01期
关键词
D O I
10.1039/b102790p
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The integration of a wet analysis system on a glass chip was demonstrated and determination of Co(II) was performed using this system. The Co(II) was extracted into m-xylene from aqueous solution as 2-nitroso-1-naphthol chelates, and colorimetric determination of the m-xylene phase was applied by a thermal lens microscope. The integration of the chemical operation procedures shown here leads to a considerable reduction in analyzing time. The time for extraction in the integrated system, 10 min, was about tenfold shorter than a conventional system using a separatory funnel and mechanical shaker. Moreover, troublesome operations such as phase separation necessary for the conventional system could be omitted. The determination of Co(II) in the range 2 x 10(-7)-1 x 10(-8) M, which was estimated to be 0.072-1.44 zmol, was achieved.
引用
收藏
页码:72 / 75
页数:4
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