Rab3A and calmodulin regulate acrosomal exocytosis by mechanisms that do not require a direct interaction

被引：26

作者：

Yunes, R

Tomes, C

Michaut, M

De Blas, G

Rodriguez, F

Regazzi, R

Mayorga, LS ^{[1
]}

机构：

[1] Univ Nacl Cuyo, Cellular & Mol Biol Lab, Inst Histol & Embriol, IHEM,CONICET,Fac Ciencias Med, RA-5500 Mendoza, Argentina

[2] Univ Lausanne, Inst Biol Cellulaire & Morphol, CH-1005 Lausanne, Switzerland

来源：

FEBS LETTERS | 2002年 / 525卷 / 1-3期

关键词：

Rab3; calmodulin; acrosomal exocytosis; permeabilized cell; human spermatozoon;

D O I：

10.1016/S0014-5793(02)03102-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction between Rab3A and calmodulin is necessary for the inhibitory effect of Rab3A in neuroendocrine cells. Contrastingly, Rab3A triggers the exocytosis known as acrosome reaction in permeabilized spermatozoa. Here we show that a Rab3A mutant that cannot bind calmodulin was fully capable of triggering acrosomal exocytosis. Additionally, calmodulin by itself abrogated the exocytosis triggered by Rab3A. The effect was observed with both the wild type protein and the calmodulin binding deficient mutant. Our results indicate that the inhibitory and stimulatory effects of Rab3A in different exocytic processes are mediated by different effectors. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：126 / 130

页数：5