Screening of sugar converting enzymes using quantitative MALDI-ToF mass spectrometry

被引：32

作者：

Bungert, D ^{[1
]}

Bastian, S ^{[1
]}

Heckmann-Pohl, DM ^{[1
]}

Giffhorn, F ^{[1
]}

Heinzle, E ^{[1
]}

Tholey, A ^{[1
]}

机构：

[1] Univ Saarland, D-66123 Saarbrucken, Germany

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 13期

关键词：

ionic liquid matrices; MALDI-ToF MS; pyranose oxidase; quantitation; screening;

D O I：

10.1023/B:BILE.0000032965.18721.62

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Quantitative matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-ToF MS) was applied for the screening of ten pyranose oxidase variants. Quantitative MALDI-ToF MS using isotopic labeled internal standards and ionic liquid matrices was performed using aliquots of enzyme reaction mixtures without prepurification steps. The results obtained were in good agreement with HPLC measurements. Analysis time was approx. 3.5 min for a five-fold determination. Thus, quantitative MALDI-ToF MS can be used as a tool for screening of sugar converting enzymes.

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收藏

页码：1025 / 1030

页数：6

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