Method for purification of fluorescence-labeled oligosaccharides by pyridylamination

被引：16

作者：

Natsuka, S ^{[1
]}

Adachi, J ^{[1
]}

Kawaguchi, M ^{[1
]}

Ichikawa, A ^{[1
]}

Ikura, K ^{[1
]}

机构：

[1] Kyoto Inst Technol, Fac Text Sci, Dept Appl Biol, Sakyo Ku, Kyoto 6068585, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2002年 / 66卷 / 05期

关键词：

pyridylamination; oligosaccharide; fluorescence; glycome;

D O I：

10.1271/bbb.66.1174

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a convenient method for purification of PA-oligosaccharides to remove contaminants originating from natural fluorescent materials, and excess reagents as well as by-products of tagging reactions in glycan analysis. The method, using a C18-cartridge, is simple and powerful to remove them. Several examples of experiments that showed the usefulness of this purification method are described in this report.

引用

页码：1174 / 1175

页数：2

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