Novel cyanine dye-labeled dideoxynucleoside triphosphates for DNA sequencing

被引：15

作者：

Duthie, RS ^{[1
]}

Kalve, IM ^{[1
]}

Samols, SB ^{[1
]}

Hamilton, S ^{[1
]}

Livshin, I ^{[1
]}

Khot, M ^{[1
]}

Nampalli, S ^{[1
]}

Kumar, S ^{[1
]}

Fuller, CW ^{[1
]}

机构：

[1] Amersham Biosci, Piscataway, NJ 08855 USA

来源：

BIOCONJUGATE CHEMISTRY | 2002年 / 13卷 / 04期

关键词：

D O I：

10.1021/bc015522y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Single color cyanine dye-labeled (Cy 5.0 and Cy 5.5) dideoxynucleoside-5'-triphosphates, or 'terminators', containing different spacer lengths were synthesized and evaluated for efficacy in DNA sequencing methods using a modified thermally stable DNA polymerase. The single color cyanine dye terminators were formulated into two separate sets of sequencing mixes, one for Cy 5.0 and the other for Cy 5.5, and evaluated on different automated sequencing platforms. Each set of mixes included two pyrimidine terminators with 17-atom linkers and two purine terminators with 10-atom linkers between the dye and the nucleotide. The two sets of cyanine dye-labeled terminators chosen for this cycle sequencing study produced improved band patterns with band uniformity similar to that obtained with dye-primer sequencing methods.

引用

页码：699 / 706

页数：8

共 20 条

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