Differentiating infection from vaccination in foot-and-mouth-disease: evaluation of an ELISA based on recombinant 3ABC

被引:46
作者
Bruderer, U
Swam, H
Haas, B
Visser, N
Brocchi, E
Grazioli, S
Esterhuysen, JJ
Vosloo, W
Forsyth, M
Aggarwal, N
Cox, S
Armstrong, R
Anderson, J
机构
[1] Intervet Int BV, NL-5830 AA Boxmeer, Netherlands
[2] Bommeli Diagnost, Liebefeld, Switzerland
[3] DLO, ID, Lelystad, Netherlands
[4] Bundesforsch Anstalt Viruskrankheiten, Inst Immunol, Tubingen, Germany
[5] Inst Zooprofilatt Sperimentale Lombardia & Emilia, Brescia, Italy
[6] Onderstepoort Vet Inst, Onderstepoort, South Africa
[7] Inst Anim Hlth, Pirbright Lab, Pirbright, England
关键词
foot-and-mouth-disease; ELISA; serology; vaccination; surveillance;
D O I
10.1016/j.vetmic.2004.01.021
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Recent devastating outbreaks of foot-and-mouth disease (FMD) in Europe have reopened the discussion about the adequacy of the non-vaccination strategy implemented by the EU in 1991. Here we describe the evaluation of a new commercially available test kit for the discrimination between vaccination and infection. The test is based on the detection of antibodies against the recombinant non-structural (NS) protein 3ABC. In contrast to immunization with vaccines free of 3ABC, these antibodies are elicited as a consequence of infection. Testing more than 3600 negative sera from several countries revealed a specificity of >99% for bovine, ovine, and porcine samples. Antibodies specific for 3ABC can be detected as soon as 10 days post-infection. As compared with the occurrence of antibodies against structural proteins of FMDV, anti-3ABC antibodies can be detected 5-10 days later, depending on the species. No anti-3ABC antibodies were detected in sera from vaccination experiments or in field sera from vaccinated animals. However, anti-3ABC antibodies can be detected in vaccinated animals upon challenge. These results provide evidence that this test can facilitate the use of vaccines in new strategies against FMD. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:187 / 197
页数:11
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