Boronate affinity monolith for highly selective enrichment of glycopeptides and glycoproteins

被引：172

作者：

Chen, Ming ^{[1
]}

Lu, Yang ^{[1
]}

Ma, Qiao ^{[1
]}

Guo, Lin ^{[1
,2
,3
]}

Feng, Yu-Qi ^{[1
]}

机构：

[1] Wuhan Univ, Dept Chem, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Peoples R China

[2] Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China

[3] Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China

来源：

ANALYST | 2009年 / 134卷 / 10期

关键词：

TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; LINKED OLIGOSACCHARIDES; HORSERADISH-PEROXIDASE; SIGNAL-TRANSDUCTION; GLYCOSYLATION; CAPILLARY; PHOSPHOPROTEOMICS; PROTEINS; SENSORS;

D O I：

10.1039/b909581k

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A novel boronate affinity monolith, poly(3-acrylamidophenylboronic acid-co-ethylene dimethacrylate) (AAPBA-co-EDMA), was prepared in 530 mu m capillaries by a one-step in situ polymerization procedure using a pre-polymerization mixture consisting of functional monomer 3-acrylamidophenylboronic acid, cross-linker ethylene dimethacrylate, porogenic solvent methanol with added poly(ethylene glycol) 20 000 (PEG 20 000) and initiator azobisisobutyronitrile (AIBN). The preparation of the monolith was optimized by investigating the ratio of functional monomer to cross-linker and the effect of poly(ethylene glycol) molecular weight. The resulting boronate monolith was used as a sorbent for polymer monolith microextraction (PMME). Using nucleosides as the testing analyte, the extraction performance of this boronate monolith towards glycol-containing compounds was examined. Finally, the boronate monolith was applied for selective enrichment of glycopeptides and glycoproteins.

引用

页码：2158 / 2164

页数：7

共 33 条

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