Development of an immunosensor for the detection of vitellogenin using impedance spectroscopy

被引：92

作者：

Darain, F

Park, DS

Park, JS

Shim, YB ^{[1
]}

机构：

[1] Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea

[2] Pusan Natl Univ, Ctr Innovat Biophys Sensor Technol, Pusan 609735, South Korea

来源：

BIOSENSORS & BIOELECTRONICS | 2004年 / 19卷 / 10期

关键词：

vitellogenin; immunosensor; impedance; QCM; conducting polymer; reagentless system;

D O I：

10.1016/j.bios.2003.11.014

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A simple and direct immunosensor for the determination of carp (Carassius auratus) vitellogenin (Vtg), a female-specific protein, has been proposed based on an antibody-captured conducting polymer-coated electrode. The monoclonal antibody specific to carp (C auratus) Vtg was immobilized by covalent coupling to the carboxylic acid group on the polymer. The antibody immobilization and antibody-antigen interaction have been demonstrated by means of quartz crystal microbalance and impedance spectroscopic techniques. The impedance change occurred at the sensor surface due to the specific immuno-interaction was utilized to determine Vtg. The sensor showed high selectivity and sensitive response to Vtg in a buffered medium without redox probe. Vtg was determined in the linear range from 1.0 to 8.0 mug/l with the standard deviation of +/-0.13 (n = 3) and the detection limit was determined to be 0.42 mug/l. This method was applied to the determination of Vtg in real male and female carp (C auratus) serum samples. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：1245 / 1252

页数：8