Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography

被引：33

作者：

de Beer, Dalene ^{[1
]}

Jerz, Gerold ^{[2
]}

Joubert, Elizabeth ^{[1
,3
]}

Wray, Victor ^{[4
]}

Winterhalter, Peter ^{[2
]}

机构：

[1] ARC Infruitec Nietvoorbij, Post Harvest & Wine Technol Div, ZA-7599 Stellenbosch, South Africa

[2] Tech Univ Carolo Wilhelmina Braunschweig, Inst Food Chem, D-38106 Braunschweig, Germany

[3] Univ Stellenbosch, Dept Food Sci, ZA-7602 Stellenbosch, South Africa

[4] Helmholtz Ctr Infect Res, Dept Biol Struct, D-38124 Braunschweig, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 19期

关键词：

Counter-current chromatography; Cyclopia subternata; Fabaceae; Isomangiferin; Mangiferin; Xanthones; NMR spectroscopy; IONIZATION MASS-SPECTROMETRY; PHENOLIC COMPOSITION; XANTHONE GLYCOSIDES; TEA; POLYPHENOLS; MANGIFERIN; INTERMEDIA; LEAVES; SPP;

D O I：

10.1016/j.chroma.2009.02.056

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1: 1 :2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：4282 / 4289

页数：8

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