N-acetyl cysteine inhibits H2O2-mediated reduction in the mineralization of MC3T3-E1 cells by down-regulating Nrf2/HO-1 pathway

被引：56

作者：

Lee, Daewoo ^{[1
,2
]}

Kook, Sung-Ho ^{[1
,2
,3
,4
]}

Ji, Hyeok ^{[1
,2
]}

Lee, Seung-Ah ^{[5
]}

Choi, Ki-Choon ^{[6
]}

Lee, Kyung-Yeol ^{[1
,2
]}

Lee, Jeong-Chae ^{[1
,2
,3
,4
]}

机构：

[1] Chonbuk Natl Univ, Inst Oral Biosci, Jeonju 54896, South Korea

[2] Chonbuk Natl Univ, Sch Dent, Jeonju 54896, South Korea

[3] Chonbuk Natl Univ, Dept Bioact Mat Sci, Jeonju 54896, South Korea

[4] Chonbuk Natl Univ, Inst Mol Biol & Genet, Jeonju 54896, South Korea

[5] Chonnam Techno Coll, Dept Nursing, Chungnam 57500, South Korea

[6] RDA, Natl Inst Anim Sci, Grassland & Forage Div, Cheonan 31002, South Korea

来源：

BMB REPORTS | 2015年 / 48卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

H2O2; N-acetyl cysteine; Nrf2; Osteoblast differentiation; Oxidative stress; OSTEOBLASTIC DIFFERENTIATION; OXIDATIVE STRESS; BONE LOSS; RADIATION; PROTECTS; SYSTEM; HO-1;

D O I：

10.5483/BMBRep.2015.48.11.112

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

There are controversial findings regarding the roles of nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway on bone metabolism under oxidative stress. We investigated how Nrf2/HO-1 pathway affects osteoblast differentiation of MC3T3-E1 cells in response to hydrogen peroxide (H2O2), N-acetyl cysteine (NAC), or both. Exposing the cells to H2O2 decreased the alkaline phosphatase activity, calcium accumulation, and expression of osteoblast markers, such as osteocalcin and runt-related transcription factor-2. In contrast, H2O2 treatment increased the expression of Nrf2 and HO-1 in the cells. Treatment with hemin, a chemical HO-1 inducer, mimicked the inhibitory effect of H2O2 on osteoblast differentiation by increasing the HO-1 expression and decreasing the osteogenic marker genes. Pretreatment with NAC restored all changes induced by H2O2 to near normal levels in the cells. Collectively, our findings suggest that H2O2-mediated activation of Nrf2/HO-1 pathway negatively regulates the osteoblast differentiation, which is inhibited by NAC.

引用

页码：636 / 641

页数：6

共 29 条

[1]

Pro-healing potential of hemin: An inducer of heme oxygenase-1 [J].

Ahanger, Azad A. ;

Prawez, Shahid ;

Leo, Marie Dennis Marcus ;

Kathirvel, Kandaswamy ;

Kumar, Dinesh ;

Tandan, Surendra K. ;

Malik, Jitendra K. .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2010, 645 (1-3) :165-170

[2]

Effects of reactive oxygen species (ROS) on antioxidant system and osteoblastic differentiation in MC3T3-E1 cells [J].

Arai, Masato ;

Shibata, Yasuko ;

Pugdee, Kamolparn ;

Abiko, Yoshimitsu ;

Ogata, Yorimasa .

IUBMB LIFE, 2007, 59 (01) :27-33

[3]

Oxidative stress inhibits osteoblastic differentiation of bone cells by ERK and NF-κB [J].

Bai, XC ;

Lu, D ;

Bai, J ;

Zheng, H ;

Ke, ZY ;

Li, XM ;

Luo, SQ .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2004, 314 (01) :197-207

[4]

Oxidative stress, free radicals and bone remodeling [J].

Banfi, Giuseppe ;

Iorio, Eugenio L. ;

Corsi, Massimiliano M. .

CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2008, 46 (11) :1550-1555

[5]

Nrf-2 mediated heme oxygenase-1 expression, an antioxidant-independent mechanism, contributes to anti-atherogenesis and vascular protective effects of Ginkgo biloba extract [J].

Chen, Jia-Shiong ;

Huang, Po-Hsun ;

Wang, Chao-Hung ;

Ling, Feng-Yen ;

Tsai, Hsiao-Ya ;

Wu, Tao-Cheng ;

Lin, Shing-Jong ;

Chen, Jaw-Wen .

ATHEROSCLEROSIS, 2011, 214 (02) :301-309

[6]

Wnt/β-catenin signaling enhances osteoblastogenic differentiation from human periodontal ligament fibroblasts [J].

Heo, Jung Sun ;

Lee, Seung-Youp ;

Lee, Jeong-Chae .

MOLECULES AND CELLS, 2010, 30 (05) :449-454

[7]

Nrf2 negatively regulates osteoblast differentiation via interfering with Runx2-dependent transcriptional activation [J].

Hinoi, Eiichi ;

Fujimori, Sayumi ;

Wang, Liyang ;

Hojo, Hironori ;

Uno, Kyosuke ;

Yoneda, Yukio .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (26) :18015-18024

[8]

Fibroblast growth factor-7 facilitates osteogenic differentiation of embryonic stem cells through the activation of ERK/Runx2 signaling [J].

Jeon, Young-Mi ;

Kook, Sung-Ho ;

Rho, Sang-Jung ;

Lim, Shin-Saeng ;

Choi, Ki-Choon ;

Kim, Hee-Soon ;

Kim, Jong-Ghee ;

Lee, Jeong-Chae .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 2013, 382 (1-2) :37-45

[9]

Ferric ion could facilitate osteoclast differentiation and bone resorption through the production of reactive oxygen species [J].

Jia, Peng ;

Xu, You Jia ;

Zhang, Zeng Li ;

Li, Kai ;

Li, Bingyan ;

Zhang, Wen ;

Yang, Huilin .

JOURNAL OF ORTHOPAEDIC RESEARCH, 2012, 30 (11) :1843-1852

[10]

N-acetylcysteine stimulates osteoblastic differentiation of mouse calvarial cells [J].

Jun, Ji Hae ;

Lee, Sun-Hwan ;

Kwak, Han Bok ;

Lee, Zang Hee ;

Seo, Sang-Beum ;

Woo, Kyung Mi ;

Ryoo, Hyun-Mo ;

Kim, Gwan-Shik ;

Baek, Jeong-Hwa .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2008, 103 (04) :1246-1255

← 1 2 3 →