Sensitive ELISA test for determination of ochratoxin A

被引：72

作者：

Barna-Vetro, I

Solti, L

Teren, J

Gyongyosi, A

Szabo, E

Wolfling, A

机构：

[1] UNIV VET SCI BUDAPEST, H-1400 BUDAPEST, HUNGARY

[2] ANIM HLTH & FOOD CONTROL STN, H-6701 SZEGED, HUNGARY

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1996年 / 44卷 / 12期

关键词：

ochratoxin A; ELISA; cereals;

D O I：

10.1021/jf960442n

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A direct, competitive enzyme-linked immunosorbent assay (ELISA) with monoclonal antibody has been developed for quantitative determination of ochratoxin A (OA) in different cereals. A dichloromethane/citric acid mixture was used for extraction of cereals. This cleanup procedure proved to be as effective for ochratoxin A extraction as protocols using strong acids. The mean within-assay and interassay coefficients of variation for the standard curve was <10%. The range of this test is 1-10 ng/g, with a detection limit of 0.5 ng/g OA. The toxin recovery from cereals infected with 5-100 ng/mL OA varied between 90 and 130%.

引用

页码：4071 / 4074

页数：4

共 17 条

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