Fgr, a major locus that modulates the fructose to glucose ratio in mature tomato fruits

被引:57
作者
Levin, I
Gilboa, N
Yeselson, E
Shen, S
Schaffer, AA
机构
[1] Agr Res Org, Volcani Ctr, Dept Genet & Plant Breeding, Inst Field & Garden Crops, IL-50250 Bet Dagan, Israel
[2] Agr Res Org, Volcani Ctr, Dept Vegetable Crops, Inst Field & Garden Crops, IL-50250 Bet Dagan, Israel
关键词
Lycopersicon hirsutum; Lycopersicon esculentum; PCR; fructose; glucose;
D O I
10.1007/s001220050034
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Agenetic trait determining the ratio of fructose to glucose in mature tomato fruits is described. A backcross breeding program based on the interspecific cross of Lycoparsicon hirsutum and L. esculentum yielded stable genotypes with a high ratio of fructose to glucose (>1.5:1) compared with the approximately equimolar ratios found in L. esculentum. Two inter-simple-sequence repeat (ISSR) DNA sequences, highly associated (20 <LOD score <21) with the trait, were identified. The markers were found to be less associated with either glucose or fructose levels individually (2 <LOD score <3) and were statistically unlinked to total sugars and total soluble solids (TSS). These two ISSR bands segregated in a dominant fashion and were found to be allelic to each other, one associated in coupling and the other in repulsion with the trait of high fructose to glucose ratio. Both ISSR markers were mapped to the centromeric region of tomato chromosome 4. Quantitative analysis of the identified locus, based on data from segregating F-2, BC and F-3 populations from the cross between genotypes having high and low fructose to glucose ratios, suggested that the L. hirsutum-derived allele (Fgr(H)), which increases the fructose to glucose ratio, is partially dominant. Fgi(H) leads to an increase in fructose levels and a subsequent decrease in glucose levels, with no effect on total hexose levels. Accordingly, we conclude that the Fgr locus modulates the partitioning of hexose sugars between fructose and glucose, with no effect on total sugars or TSS.
引用
收藏
页码:256 / 262
页数:7
相关论文
共 24 条
[1]   Carbohydrate studies I. The relative sweetness of pure sugars [J].
Biester, A ;
Wood, MW ;
Wahlin, CS .
AMERICAN JOURNAL OF PHYSIOLOGY, 1925, 73 (02) :387-396
[2]   INTROGRESSION INTO TOMATO (LYCOPERSICON-ESCULENTUM) OF THE L-CHMIELEWSKII SUCROSE ACCUMULATOR GENE (SUCR) CONTROLLING FRUIT SUGAR COMPOSITION [J].
CHETELAT, RT ;
DEVERNA, JW ;
BENNETT, AB .
THEORETICAL AND APPLIED GENETICS, 1995, 91 (02) :327-333
[3]   OCCURRENCE OF SUCROSE IN FRUIT OF SOME SPECIES OF LYCOPERSICON [J].
DAVIES, JN .
NATURE, 1966, 209 (5023) :640-&
[4]   THE CONSTITUENTS OF TOMATO FRUIT - THE INFLUENCE OF ENVIRONMENT, NUTRITION, AND GENOTYPE [J].
DAVIES, JN ;
HOBSON, GE .
CRC CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 1981, 15 (03) :205-280
[5]   Lycopersicon esculentum lines containing small overlapping introgressions from L. pennellii [J].
Eshed, Y. ;
Abu-Abied, M. ;
Saranga, Y. ;
Zamir, D. .
THEORETICAL AND APPLIED GENETICS, 1992, 83 (08) :1027-1034
[6]   INTROGRESSIONS FROM LYCOPERSICON PENNELLII CAN IMPROVE THE SOLUBLE SOLIDS YIELD OF TOMATO HYBRIDS [J].
ESHED, Y ;
ZAMIR, D .
THEORETICAL AND APPLIED GENETICS, 1994, 88 (6-7) :891-897
[7]   A GENOMIC LIBRARY OF LYCOPERSICON PENNELLII IN LYCOPERSICON-ESCULENTUM - A TOOL FOR FINE MAPPING OF GENES [J].
ESHED, Y ;
ZAMIR, D .
EUPHYTICA, 1994, 79 (03) :175-179
[8]   MICROPREP PROTOCOL FOR EXTRACTION OF DNA FROM TOMATO AND OTHER HERBACEOUS PLANTS [J].
FULTON, TM ;
CHUNWONGSE, J ;
TANKSLEY, SD .
PLANT MOLECULAR BIOLOGY REPORTER, 1995, 13 (03) :207-209
[9]   AMPLIFICATION OF DNA MARKERS FROM EVOLUTIONARILY DIVERSE GENOMES USING SINGLE PRIMERS OF SIMPLE-SEQUENCE REPEATS [J].
GUPTA, M ;
CHYI, YS ;
ROMEROSEVERSON, J ;
OWEN, JL .
THEORETICAL AND APPLIED GENETICS, 1994, 89 (7-8) :998-1006
[10]   PCR-GENERATED MOLECULAR MARKERS FOR THE INVERTASE GENE AND SUCROSE ACCUMULATION IN TOMATO [J].
HADAS, R ;
SCHAFFER, A ;
MIRON, D ;
FOGELMAN, M ;
GRANOT, D .
THEORETICAL AND APPLIED GENETICS, 1995, 90 (7-8) :1142-1148