Activation of HTLV-1 transcription in the presence of tax is independent of the acetylation of CREB-2 (ATF-4)

被引:24
作者
Gachon, F [1 ]
Devaux, C [1 ]
Mesnard, JM [1 ]
机构
[1] Univ Montpellier 1, Lab Infect Retrovirales & Signalisat Cellulaire, CNRS, EP 2104, F-34060 Montpellier, France
关键词
D O I
10.1006/viro.2002.1501
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have previously demonstrated that the bZIP transcription factor CREB-2, also called ATF-4, trans-activates, in association with the viral protein Tax, the human T-cell leukemia virus type I (HTLV-1) promoter. In this study, we have examined whether CREB-2 acetylation affects transcriptional activation mediated by Tax. We present evidence that CREB-2 is acetylated in vitro and in vivo. CREB-2 is acetylated in two regions: the basic domain of the bZIP (from amino acid residue 270 to 300) and the short basic domain (from 342 to 351) located downstream from the bZIP. We also demonstrate that CREB-2 is acetylated by p300/CBP but not by p/CAF. Moreover, replacement of lysine by arginine in the basic domains decreases the trans-activating capacity of CREB-2. However, in the presence of Tax, the HTLV-1 transcription remains fully activated by these CREB-2 mutants. Although we cannot totally exclude that the mutations could also affect CREB-2 structure and activity independent of acetylation, our results suggest that activation of the viral promoter in the presence of Tax is independent of the CREB-2 acetylation. (C) 2002 Elsevier Science (USA).
引用
收藏
页码:271 / 278
页数:8
相关论文
共 42 条
[11]   FUNCTIONAL ACTIVATION OF THE LONG TERMINAL REPEAT OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I BY A TRANS-ACTING FACTOR [J].
FUJISAWA, J ;
SEIKI, M ;
KIYOKAWA, T ;
YOSHIDA, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (08) :2277-2281
[12]   A TRANSCRIPTIONAL ENHANCER SEQUENCE OF HTLV-I IS RESPONSIBLE FOR TRANSACTIVATION MEDIATED BY P40X OF HTLV-I [J].
FUJISAWA, J ;
SEIKI, M ;
SATO, M ;
YOSHIDA, M .
EMBO JOURNAL, 1986, 5 (04) :713-718
[13]   The cAMP response element binding protein-2 (CREB-2) can interact with the C/EBP-homologous protein (CHOP) [J].
Gachon, F ;
Gaudray, G ;
Thébault, S ;
Basbous, J ;
Koffi, JA ;
Devaux, C ;
Mesnard, JM .
FEBS LETTERS, 2001, 502 (1-2) :57-62
[14]   CREB-2, a cellular CRE-Dependent transcription repressor, functions in association with tax as an activator of the human T-cell leukemia virus type 1 promoter [J].
Gachon, F ;
Peleraux, A ;
Thebault, S ;
Dick, J ;
Lemasson, I ;
Devaux, C ;
Mesnard, JM .
JOURNAL OF VIROLOGY, 1998, 72 (10) :8332-8337
[15]   Molecular interactions involved in the transactivation of the human T-cell leukemia virus type 1 promoter mediated by Tax and CREB-2 (ATF-4) [J].
Gachon, F ;
Thebault, S ;
Peleraux, A ;
Devaux, C ;
Mesnard, JM .
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (10) :3470-3481
[16]   Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain [J].
Gu, W ;
Roeder, RG .
CELL, 1997, 90 (04) :595-606
[17]   CROSS-FAMILY DIMERIZATION OF TRANSCRIPTION FACTORS FOS JUN AND ATF CREB ALTERS DNA-BINDING SPECIFICITY [J].
HAI, T ;
CURRAN, T .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (09) :3720-3724
[18]   The molecular biology and nomenclature of the activating transcription factor/cAMP responsive element binding family of transcription factors: activating transcription factor proteins and homeostasis [J].
Hai, T ;
Hartman, MG .
GENE, 2001, 273 (01) :1-11
[19]   TRANSCRIPTION FACTOR ATF CDNA CLONES - AN EXTENSIVE FAMILY OF LEUCINE ZIPPER PROTEINS ABLE TO SELECTIVELY FORM DNA-BINDING HETERODIMERS [J].
HAI, TW ;
LIU, F ;
COUKOS, WJ ;
GREEN, MR .
GENES & DEVELOPMENT, 1989, 3 (12B) :2083-2090
[20]  
Jiang H, 1999, MOL CELL BIOL, V19, P8136