Expression of voltage-gated K+ channels in human atrium

Voltage-gated K+ channels underlie repolarisation of the cardiac action potential and represent a potential therapeutic target in the treatment of cardiac dysrhythmias. However, very little is known about the relative expression of K+ channel subunits in the human myocardium. We used a semi-quantitative RT-PCR technique to examine the relative expression of mRNAs for the voltage-gated K+ channel subunits, Kv1.2, Kv1.4, Kv1.5, Kv2.1, Kv4.2, Kv4.3, KvLQT1, HERG and IsK in samples of human atrial appendage. Data were expressed as a percentage expression density relative to an 18S ribosomal RNA internal standard. The most abundant K+ channel mRNAs were Kv4.3 (80.7 +/- 10.1 %), Kv1.5 (69.7 +/- 11.2 %) and HERG (55.9 +/- 21.5 %). Significant expression of KvLQT1 (33.5 +/- 5.5 %,) and Kvl.4 (26.7 +/- 9.6%) was also detected. Levels of mRNAs for Kv1.2 and IsK were very low and neither Kv2.1 nor Kv4.2 mRNA were detected in any experiments. Whole-cell patch-clamp techniques were used to examine the outward currents of isolated human atrial myocytes at 37 degreesC. These recordings demonstrated the existence of transient (I-to1), and sustained (t(so)) outward currents in isolated human atrial myocytes. I-to1, and not I-so, showed voltage-dependent inactivation during 100 ms pre-pulses. Both I-to1 and I-so were inhibited by high concentrations (2 mM) of the K+ channel blocker, 4-aminopyridine (4-AP). However, lower concentrations of 4-AP (10 muM) inhibited I-so selectively. I-to1 recovered from inactivation relatively rapidly (tau similar to21 ms). These data, with published information regarding the properties of expressed K+ channels, suggest that Kv4.3 represents the predominant K+ channel subunit underlying I-to1 with little contribution of Kv1.4. The sensitivity of I-so to very low concentrations of 4-aminopyridine and the relatively low expression of mRNA for Kv1.2 and Kv2.1 is consistent with the major contribution of KvI.5 to this current. The physiological significance of the expression of KvLQT1 and KvIA mRNA in the human atrium warrants further investigation.