Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F1-ATPase by controlled dehydration

被引：29

作者：

Bowler, Matthew W.

Montgomery, Martin G.

Leslie, Andrew G. W.

Walker, John E. ^{[1
]}

机构：

[1] MRC, Dunn Human Nutr Unit, Cambridge, England

[2] MRC, Mol Biol Lab, Cambridge, England

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2006年 / 62卷

基金：

英国医学研究理事会;

关键词：

D O I：

10.1107/S0907444906020877

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Orthorhombic crystals of bovine F-1-ATPase have been subjected to controlled dehydration. A decrease in the relative humidity surrounding the crystals to 90% reproducibly reduced their unit-cell volume by 22% (950 000 angstrom(3)) and improved the diffraction limit and mosaic spread of the crystals significantly. These dehydrated crystals diffracted X--rays to 1.8 angstrom resolution at a synchrotron source, the best diffraction limit yet attained with these crystals, although radiation damage limited the resolution of a complete data set to 1.95 angstrom.

引用

收藏

页码：991 / 995

页数：5

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