Reproducible improvements in order and diffraction limit of crystals of bovine mitochondrial F1-ATPase by controlled dehydration

被引:29
作者
Bowler, Matthew W.
Montgomery, Martin G.
Leslie, Andrew G. W.
Walker, John E. [1 ]
机构
[1] MRC, Dunn Human Nutr Unit, Cambridge, England
[2] MRC, Mol Biol Lab, Cambridge, England
来源
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2006年 / 62卷
基金
英国医学研究理事会;
关键词
D O I
10.1107/S0907444906020877
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Orthorhombic crystals of bovine F-1-ATPase have been subjected to controlled dehydration. A decrease in the relative humidity surrounding the crystals to 90% reproducibly reduced their unit-cell volume by 22% (950 000 angstrom(3)) and improved the diffraction limit and mosaic spread of the crystals significantly. These dehydrated crystals diffracted X--rays to 1.8 angstrom resolution at a synchrotron source, the best diffraction limit yet attained with these crystals, although radiation damage limited the resolution of a complete data set to 1.95 angstrom.
引用
收藏
页码:991 / 995
页数:5
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