MicroRNA expression profiling during neural differentiation of mouse embryonic carcinoma P19 cells

被引：19

作者：

Huang, Bing ^{[1
]}

Li, Wei ^{[1
]}

Zhao, Botao ^{[1
]}

Xia, Caihong ^{[2
]}

Liang, Ruqiang ^{[1
]}

Ruan, Kangcheng ^{[1
]}

Jing, Naihe ^{[2
]}

Jin, Youxin ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Mol Cell Biol, Shanghai 200031, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2009年 / 41卷 / 03期

基金：

中国国家自然科学基金;

关键词：

microRNA; embryonic carcinoma P19 cell; neural stem cells; neural differentiation; STEM-CELLS; NEURONAL DIFFERENTIATION; IDENTIFICATION; NETWORK;

D O I：

10.1093/abbs/gmp006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (or miRNAs) are small non-coding RNAs (21-25 nucleotides) that are involved in a wide range of activities related to the development and differentiation of cells. Comparison of the miRNA expression profiles of mouse P19 embryonic carcinoma cells with those of differentiated neural stem cells showed that the expression level of 65 miRNAs changed (2-fold) after differentiation. MiR-124a was dramatically up-regulated (more than 20-fold) while miRNAs of the miR-302 family and those in the miR-290-295 cluster were strongly down-regulated. Further analysis revealed that some important factors such as Oct4 and Sox2 appeared to be involved in the regulation of these miRNAs. These results may contribute to a better understanding of miRNA-regulated neural differentiation in early mouse embryos.

引用

页码：231 / 236

页数：6

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