Measurements of multiphoton action cross sections for multiphoton microscopy

被引:124
作者
Cheng, Li-Chung [1 ,2 ]
Horton, Nicholas G. [2 ]
Wang, Ke [3 ]
Chen, Shean-Jen [4 ,5 ]
Xu, Chris [2 ]
机构
[1] Natl Cheng Kung Univ, Dept Photon, Tainan 701, Taiwan
[2] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14853 USA
[3] Shenzhen Univ, Coll Optoelect Engn, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Shenzhen 518060, Peoples R China
[4] Natl Cheng Kung Univ, Dept Engn Sci, Tainan 701, Taiwan
[5] Natl Cheng Kung Univ, Adv Optoelect Technol Ctr, Tainan 701, Taiwan
来源
BIOMEDICAL OPTICS EXPRESS | 2014年 / 5卷 / 10期
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
2-PHOTON; BRIGHTNESS; SPECTRA;
D O I
10.1364/BOE.5.003427
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We report quantitative measurements of two-, three-, and four-photon excitation action cross sections of several commonly used fluorophores and fluorescent proteins at three different excitation wavelengths of 800 nm, 1300 nm, and 1680 nm. The measured cross section values are consistent with simple quantum mechanic estimations. These values indicate that the optimum repetition rate for deep tissue 3-photon microscopy is approximately 1 to 2 MHz. We further demonstrate that it is feasible to perform 4-photon fluorescence microscopy of GFP labeled microglia in mouse brain in vivo at 1700 nm. 4-photon excitation increases the accessibility of fluorophores at the long wavelength spectral window of 1700 nm. (C)2014 Optical Society of America
引用
收藏
页码:3427 / 3433
页数:7
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