Manganese supplementation relieves the phenotypic deficits seen in superoxide-dismutase-null Escherichia coli

被引：47

作者：

Al-Maghrebi, M

Fridovich, I

Benov, L

机构：

[1] Kuwait Univ, Dept Biochem, Fac Med, Safat 13110, Kuwait

[2] Duke Univ, Dept Biochem, Med Ctr, Durham, NC 27710 USA

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 2002年 / 402卷 / 01期

关键词：

manganese in Escherichia coli; superoxide scavenging by manganese; SOD mutants; complementation by Mn(II); aconitase; protection by Mn(II);

D O I：

10.1016/S0003-9861(02)00065-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Escherichia coli, lacking cytoplasmic superoxide dismutases, exhibits a variety of oxygen-dependent phenotypic deficits. Enrichment of the growth medium with Mn(II) relieved those deficits. Extracts of cells grown on Mn(II)-rich medium exhibited superoxide dismutase-like activity that was due partially to low-molecular-weight and partially to high-molecular-weight complexes. The high-molecular-weight activity was sensitive to proteolysis. Hence this activity is likely associated with low-affinity binding of Mn to proteins. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：104 / 109

页数：6

共 24 条

[11] The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen [J].