A Suite of Transgenic Driver and Reporter Mouse Lines with Enhanced Brain-Cell-Type Targeting and Functionality

被引：495

作者：

Daigle, Tanya L. ^{[1
]}

Madisen, Linda ^{[1
]}

Hage, Travis A. ^{[1
]}

Valley, Matthew T. ^{[1
]}

Knoblich, Ulf ^{[1
]}

Larsen, Rylan S. ^{[1
]}

Takeno, Marc M. ^{[1
]}

Huang, Lawrence ^{[1
]}

Gu, Hong ^{[1
]}

Larsen, Rachael ^{[1
]}

Mills, Maya ^{[1
]}

Bosma-Moody, Alice ^{[1
]}

Siverts, La' Akea ^{[1
]}

Walker, Miranda ^{[1
]}

Graybuck, Lucas T. ^{[1
]}

Yao, Zizhen ^{[1
]}

Fong, Olivia ^{[1
]}

Thuc Nghi Nguyen ^{[1
]}

Garren, Emma ^{[1
]}

Lenz, Garreck H. ^{[1
]}

Chavarha, Mariya ^{[2
,3
]}

Pendergraft, Julie ^{[1
]}

Harrington, James ^{[1
]}

Hirokawa, Karla E. ^{[1
]}

Harris, Julie A. ^{[1
]}

Nicovich, Philip R. ^{[1
]}

McGraw, Medea J. ^{[1
]}

Ollerenshaw, Douglas R. ^{[1
]}

Smith, Kimberly A. ^{[1
]}

Baker, Christopher A. ^{[1
]}

Ting, Jonathan T. ^{[1
]}

Sunkin, Susan M. ^{[1
]}

Lecoq, Jerome ^{[1
]}

Lin, Michael Z. ^{[2
,3
]}

Boyden, Edward S. ^{[4
,5
,6
,7
]}

Murphy, Gabe J. ^{[1
]}

da Costa, Nuno M. ^{[1
]}

Waters, Jack ^{[1
]}

Li, Lu ^{[1
]}

Tasic, Bosiljka ^{[1
]}

Zeng, Hongkui ^{[1
]}

机构：

[1] Allen Inst Brain Sci, Seattle, WA 98109 USA

[2] Stanford Univ, Sch Med, Dept Neurobiol, Stanford, CA 94305 USA

[3] Stanford Univ, Sch Med, Dept Bioengn, Stanford, CA 94305 USA

[4] MIT, MIT Media Lab, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[5] MIT, McGovern Inst, Dept Biol Engn, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[6] MIT, MIT Media Lab, Dept Brain & Cognit Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

[7] MIT, McGovern Inst, Dept Brain & Cognit Sci, 77 Massachusetts Ave, Cambridge, MA 02139 USA

来源：

CELL | 2018年 / 174卷 / 02期

关键词：

NEURAL CIRCUITS; IN-VIVO; ADULT; MICE; CRE; ORGANIZATION; ACTIVATION; EXCITATION; NEURONS; VOLTAGE;

D O I：

10.1016/j.cell.2018.06.035

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Modern genetic approaches are powerful in providing access to diverse cell types in the brain and facilitating the study of their function. Here, we report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targetedinsertion transgenesis in a wide range of neuronal types, along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain.

引用

页码：465 / +

页数：38

共 38 条

[1]

The Cellular and Synaptic Architecture of the Mechanosensory Dorsal Horn [J].

Abraira, Victoria E. ;

Kuehn, Emily D. ;

Chirila, Anda M. ;

Springel, Mark W. ;

Toliver, Alexis A. ;

Zimmerman, Amanda L. ;

Orefice, Lauren L. ;

Boyle, Kieran A. ;

Bai, Ling ;

Song, Bryan J. ;

Bashista, Karleena A. ;

O'Neill, Thomas G. ;

Zhuo, Justin ;

Tsan, Connie ;

Hoynoski, Jessica ;

Rutlin, Michael ;

Kus, Laura ;

Niederkofler, Vera ;

Watanabe, Masahiko ;

Dymecki, Susan M. ;

Nelson, Sacha B. ;

Heintz, Nathaniel ;

Hughes, David I. ;

Ginty, David D. .

CELL, 2017, 168 (1-2) :295-+

[2]

Cellular resolution circuit mapping with temporal-focused excitation of soma targeted channelrhodopsin [J].

Baker, Christopher A. ;

Elyadat, Yishai M. ;

Parra, Andres ;

Bolton, M. McLean .

ELIFE, 2016, 5

[3]

An R-CaMP1.07 reporter mouse for cell-typespecific expression of a sensitive red fluorescent calcium indicator [J].

Bethge, Philipp ;

Carta, Stefano ;

Lorenzo, Dayra A. ;

Egolf, Ladan ;

Goniotaki, Despoina ;

Madisen, Linda ;

Voigt, Fabian F. ;

Chen, Jerry L. ;

Schneider, Bernard ;

Ohkura, Masamichi ;

Nakai, Junichi ;

Zeng, Hongkui ;

Aguzzi, Adriano ;

Helmchen, Fritjof .

PLOS ONE, 2017, 12 (06)

[4]

Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators [J].

Chamberland, Simon ;

Yang, Helen H. ;

Pan, Micheal M. ;

Evans, Stephen W. ;

Guan, Sihui ;

Chavarha, Mariya ;

Yang, Ying ;

Salesse, Charleen ;

Wu, Haodi ;

Wu, Joseph C. ;

Clandinin, Thomas R. ;

Toth, Katalin ;

Lin, Michael Z. ;

St-Pierre, Francois .

ELIFE, 2017, 6

[5]

Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

[6]

MAPPING CELL FATE AND FUNCTION USING RECOMBINASE-BASED INTERSECTIONAL STRATEGIES [J].

Dymecki, Susan M. ;

Ray, Russell S. ;

Kim, Jun C. .

METHODS IN ENZYMOLOGY, VOL 477: GUIDE TO TECHNIQUES IN MOUSE DEVELOPMENT, PART B: MOUSE MOLECULAR GENETICS, SECOND EDITION, 2010, 477 :183-213

[7]

Developmental and adult phenotyping directly from mutant embryonic stem cells [J].

George, Sophia H. L. ;

Gertsenstein, Marina ;

Vintersten, Kristina ;

Korets-Smith, Ella ;

Murphy, John ;

Stevens, Mary E. ;

Haigh, Jody J. ;

Nagy, Andras .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (11) :4455-4460

[8]

Strain Background Influences Neurotoxicity and Behavioral Abnormalities in Mice Expressing the Tetracycline Transactivator [J].

Han, Harry J. ;

Allen, Carolyn C. ;

Buchovecky, Christie M. ;

Yetman, Michael J. ;

Born, Heather A. ;

Marin, Miguel A. ;

Rodgers, Shaefali P. ;

Song, Bryan J. ;

Lu, Hui-Chen ;

Justice, Monica J. ;

Probst, Frank J. ;

Jankowsky, Joanna L. .

JOURNAL OF NEUROSCIENCE, 2012, 32 (31) :10574-10586

[9]

Anatomical characterization of Cre driver mice for neural circuit mapping and manipulation [J].

Harris, Julie A. ;

Hirokawa, Karla E. ;

Sorensen, Staci A. ;

Gu, Hong ;

Mills, Maya ;

Ng, Lydia L. ;

Bohn, Phillip ;

Mortrud, Marty ;

Ouellette, Benjamin ;

Kidney, Jolene ;

Smith, Kimberly A. ;

Dang, Chinh ;

Sunkin, Susan ;

Bernard, Amy ;

Oh, Seung Wook ;

Madisen, Linda ;

Zeng, Hongkui .

FRONTIERS IN NEURAL CIRCUITS, 2014, 8

[10]

Local processing in neurites of VGluT3-expressing amacrine cells differentially organizes visual information [J].

Hsiang, Jen-Chun ;

Johnson, Keith P. ;

Madisen, Linda ;

Zeng, Hongkui ;

Kerschensteiner, Daniel .

ELIFE, 2017, 6

← 1 2 3 4 →